Coding
Api

Part:BBa_K2834000:Design

Designed by: Ana Laura Ortega Ceniceros   Group: iGEM18_Tec-Chihuahua   (2018-10-07)


Apidaecin bee antimicrobial peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence does not encode a start codon nor a stop codon, however, it is integrated with a composite that encodes fusion peptides upstream and downstream of apidaecin, enabling the proper traduction of the final fused peptide.


Source

Amino acid sequence retrieved from Chen, X., Li, J., Sun, H., Li, S., Chen, T., Liu, G., & Dyson, P. (2017). High-level heterologous production and Functional Secretion by recombinant Pichia pastoris of the shortest proline-rich antibacterial honeybee peptide Apidaecin. Scientific Reports, 7(1). doi:10.1038/s41598-017-15149-3

References